CALL FOR PAPERS Comparative Genomics Genomic profiling of developing cardiomyocytes from recombinant murine embryonic stem cells reveals regulation of transcription factor clusters
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چکیده
Michael J. Seewald,* Peter Ellinghaus,* Astrid Kassner, Ines Stork, Martina Barg, Sylvia Niebrügge, Stefan Golz, Holger Summer, Robert Zweigerdt, Eva-Maria Schräder, Samantha Feicht, Kornelia Jaquet, Stephanie Reis, Reiner Körfer, and Hendrik Milting Department of Target Discovery, Bayer Healthcare AG, Wuppertal; Herzund Diabeteszentrum Nordrhein-Westfalen, Erich und Hanna Klessmann-Institut für Kardiovaskuläre Forschung und Entwicklung, Bad Oeynhausen, Germany; Stem Cell Bioengineering Laboratory; University of Toronto; Terrence Donnelly Centre for Cellular and Biomolecular Research; Toronto, Ontario, Canada; Institute of Medical Biology, Singapore; and Abteilung für Molekulare Kardiologie, Berufsgenossenschaftliche Kliniken Bergmannsheil, Bochum, Germany
منابع مشابه
Genomic profiling of developing cardiomyocytes from recombinant murine embryonic stem cells reveals regulation of transcription factor clusters.
Cardiomyocytes derived from pluripotent embryonic stem cells (ESC) have the advantage of providing a source for standardized cell cultures. However, little is known on the regulation of the genome during differentiation of ESC to cardiomyocytes. Here, we characterize the transcriptome of the mouse ESC line CM7/1 during differentiation into beating cardiomyocytes and compare the gene expression ...
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Stem cells are a good alternative for regenerative medicine because of their characteristics such as self-renewal and differentiation potential. They are classified into different types of stem cells including embryonic stem cells, induced pluripotent stem cells, multipotent stem cells, and ultimately uni-potent stem cells. Mesenchymal stem cells extracted from adult tissues. Due to the lack of...
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Purpose: To investigate the effect of cardiogel and matrigel on the ultrastructure of embryonic stem cell-derived cardiomyocytes. ECM: Extracellular Matrix derived from cardiac fibroblasts (cardiogel), commercial extracellular matrix (matrigel) and control group (without ECM) were cultured for up to 21 days. Ultrastructural properties of cardiomyocytes were evaluated by transmitting electron mi...
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It is now well documented that human embryonic stem cells (hESCs) can differentiate into functional cardiomyocytes. These cells constitute a promising source of material for use in drug development, toxicity testing, and regenerative medicine. To assess their utility as replacement or complement to existing models, extensive phenotypic characterization of the cells is required. In the present s...
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Purpose: We investigated the effect of (bFGF) (basic-Fibroblast Growth Factor) on the differentiation of divided cardiomyocytes from mouse embryonic stem cells (ES) and their pharmacological properties. Materials and Methods: The mouse embryonic stem cells (Royan B1) were cultured as 800 cells per 20µl of a hanging drop. After two days, ES cells in each drop aggregated to form embryoid bodies ...
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تاریخ انتشار 2009